Kitt Peak National Observatory (KPNO) is an optical observatory at an altitude of 2,120 m on Kitt Peak in the Quinlan Mountains 90 m southwest of Tucson, Arizona. Its main instruments are the 4-m Mayall Telescope and the 3.5-m WIYN Telescope.

Also on Kitt Peak are the McMath-Pierce Solar Telescope and the Solar Vacuum Tower, operated by the National Solar Observatory, and various instruments belonging to the Steward Observatory.